期刊简介
本刊刊登基础医学、预防医学与相关的科技新理念、新成果、新技术等研究论文和医学管理方面论文。
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首页>中国现代医学杂志
- 杂志名称：中国现代医学杂志
- 主管单位：中华人民共和国教育部
- 主办单位：中南大学,中南大学肝胆肠外科研究中心
- 国际刊号：1005-8982
- 国内刊号：43-1225/R
- 出版周期：旬刊
期刊荣誉：2000年、2002年二次被省科技厅、省新闻出版局评为一级期刊期刊收录：CSCD 中国科学引文数据库来源期刊(含扩展版), 维普收录(中), 万方收录(中), 哥白尼索引(波兰), 国家图书馆馆藏, CA 化学文摘(美), 北大核心期刊(中国人文社会科学核心期刊), 知网收录(中), 上海图书馆馆藏, 统计源核心期刊(中国科技论文核心期刊)
关键词：Neural Stem/Progenitor Cell, Isolation and Expansion, human, cortex
摘要：Objective: To isolate, culture and identify human embryonic neural stem cells and to establish a practical passaging method. Method:The cerebral cortex cells were isolated from aborted embryos (11～13 weeks)by mechanical dissociation, and cultured in DMEM/F12 culture medium supplemented with N2 and growth factors for proliferation. Upon passaging, the neurospheres were pipetted gentlely to separate them into several cell masses and then grown in growth medium. The cells were grown in DMEM/F12 medium with serum (without growth factors) to induce differentiation. The stem cell, neuron, astrocyte and oligodendrocyte were identified by immunocytochemistry with antibodies to vimentin, MAP2, GFAP and GalC, respectively. Results:The primary cells grew together and formed neurospheres at 5th～7th day. They were all vimentin positive and could be passaged for at least 8passages. After passaging, the cell masses grew up and formed new neurospheres rapidly. These cells could differentiated into MAP2 ( + ), GFAP( + ) or GalC( + ) cells. Conclusion: The neural stem cells from human embryonic cerebral cortex have the capacity of proliferation and multi - differentiation in vitro. The passaging methods we used in this experiment were practical and convenient.
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